The teen today posted a photo of her sister Khloe pictured with who is believed to be their mother Kris Jenner's hairdresser, Alex Roldan, with the accompanying message: 'First official photo of my sister and her dad! Like father like daughter!'
It comes following hurtful allegations last month - which Khloe strenuously denied - claiming she was not a real Kardashian and was not fathered by Robert Kardashian.
What a Twit: Kylie Jenner posted a picture of sister Khloe and who is believed to be their mother Kris's hairdresser Alex Roldan, joking it was Khloe's real father
Caustic sense of humour: She posted the accompanying message: 'First official photo of my sister and her dad! Like father like daughter!'
Kylie's tweet provoked shock from her followers.
chrissynicole04xox responded to the tweet: '@kylieejennerr pleaseeee tell us if this is real or not. Haha our heads are about to explode.'
Making her mark: Kylie pictured earlier this week in Hollywood at the premiere of The Vow
Just kidding: Kylie later clarified she was only joking
A spokesman for Kylie told MailOnline the tweet was intended as 'joke'. And now Kylie has taken to Twitter again, adding: 'It was a joke everyone! Lol!!'
Daddy' girl: Khloe in an old family portrait with her late father Robert
The 27-year-old reality star lashed out at her father Robert's ex-wfie Jan Ashley and widow Ellen, after they both verified Robert had told them he was not Khloe's father.
It came after her mother Kris admitted to being unfaithful during her marriage to Robert in her memoirs.
In the ensuing media storm, there were wild claims Khloe was fathered by Roldan - her mother's hairdresser - and former NFL star OJ Simpson's name was also touted.
Robert famously represented his friend Simpson in the infamous Nineties murder trial of the NFL star's wife Nicole Brown Simpson and her lover Ronald Goldman, in which he was eventually acquitted of the criminal charges.
Kylie Jenner tweets images from a video shoot
Family time: Another picture shows Khloe and Rob posing together
Ride with me: Another shot see's Kylie's eldest sister putting her foot up on the wheel
Robert died aged 59 in 2003 after losing his battle to cancer.